![]() While it easy to identify files that are completely misssing, this could have also caused the more cryptic problem of files incompletely downloading. Using the name of a file that I knew failed, I found that wget cryptically returns the message “No such file” or “No such directory” when a download fails, which doesn’t make a whole lot of sense because how did it know to search for that file or directory in its recursive search if it does not exist. Returning to the log files contained the wget output, I re-confirmed that the files did not contain the words error or fail (e.g. We confirmed this suspicion by checking the Genomics Core FTP site, which contained the proper 96 files in each lane. ![]() Thus we suspected there was some error in the download process. It was missing some files as well, but they were different from those missing on the cluster. Furthermore, this was not a problem with the processing pipeline the original fastq files were missing! We checked the data that had been independently downloaded to an external hard drive. When performing QC by lane, we noticed that not every lane of single cell sequencing data had 96 fastq files. Various methods of code obfuscation, mutation and virtualization will help protect your application from hacking, modifying, disassembling, illegal. others to reconstruct or reproduce the registration key generator for your software. Top Software Keywords Show more Show less
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